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Cardiac Tissue Engineering

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Milica Radisic

    1. Department Chemical Engineering&Applied Chemistry, University of Toronto, Toronto, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Lauren D. Black III

    1. Department of Biomedical Engineering, Tufts University, Medford, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1181)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Second Generation Codon Optimized Minicircle (CoMiC) for Nonviral Reprogramming of Human Adult Fibroblasts

    • Sebastian Diecke, Leszek Lisowski, Nigel G. Kooreman, Joseph C. Wu
    Pages 1-13

  3. Scalable Cardiac Differentiation of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates

    • Celine L. Bauwens, Mark D. Ungrin
    Pages 15-25

  4. Preparation and Characterization of Circulating Angiogenic Cells for Tissue Engineering Applications

    • Aleksandra Ostojic, Suzanne Crowe, Brian McNeill, Marc Ruel, Erik J. Suuronen
    Pages 27-38

  5. Isolation and Expansion of C-Kit-Positive Cardiac Progenitor Cells by Magnetic Cell Sorting

    • Kristin M. French, Michael E. Davis
    Pages 39-50

  6. Synthesis of Aliphatic Polyester Hydrogel for Cardiac Tissue Engineering

    • Sanjiv Dhingra, Richard D. Weisel, Ren-Ke Li
    Pages 51-59

  7. Fabrication of PEGylated Fibrinogen: A Versatile Injectable Hydrogel Biomaterial

    • Iris Mironi-Harpaz, Alexandra Berdichevski, Dror Seliktar
    Pages 61-68

  8. Natural Cardiac Extracellular Matrix Hydrogels for Cultivation of Human Stem Cell-Derived Cardiomyocytes

    • Donald O. Freytes, John D. O’Neill, Yi Duan-Arnold, Emily A. Wrona, Gordana Vunjak-Novakovic
    Pages 69-81

  9. Magnetically Actuated Alginate Scaffold: A Novel Platform for Promoting Tissue Organization and Vascularization

    • Yulia Sapir, Emil Ruvinov, Boris Polyak, Smadar Cohen
    Pages 83-95

  10. Shrink-Induced Biomimetic Wrinkled Substrates for Functional Cardiac Cell Alignment and Culture

    • Nicole Mendoza, Roger Tu, Aaron Chen, Eugene Lee, Michelle Khine
    Pages 97-108

  11. Injectable ECM Scaffolds for Cardiac Repair

    • Todd D. Johnson, Rebecca L. Braden, Karen L. Christman
    Pages 109-120

  12. Generation of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT)

    • Sebastian Schaaf, Alexandra Eder, Ingra Vollert, Andrea Stöhr, Arne Hansen, Thomas Eschenhagen
    Pages 121-129

  13. Cell Tri-Culture for Cardiac Vascularization

    • Ayelet Lesman, Lior Gepstein, Shulamit Levenberg
    Pages 131-137

  14. Cell Sheet Technology for Cardiac Tissue Engineering

    • Yuji Haraguchi, Tatsuya Shimizu, Katsuhisa Matsuura, Hidekazu Sekine, Nobuyuki Tanaka, Kenjiro Tadakuma et al.
    Pages 139-155

  15. Design and Fabrication of Biological Wires

    • Jason W. Miklas, Sara S. Nunes, Boyang Zhang, Milica Radisic
    Pages 157-165

  16. Collagen-Based Engineered Heart Muscle

    • Malte Tiburcy, Tim Meyer, Poh Loong Soong, Wolfram-Hubertus Zimmermann
    Pages 167-176

  17. Creation of a Bioreactor for the Application of Variable Amplitude Mechanical Stimulation of Fibrin Gel-Based Engineered Cardiac Tissue

    • Kathy Y. Morgan, Lauren D. Black III
    Pages 177-187

  18. Preparation of Acellular Myocardial Scaffolds with Well-Preserved Cardiomyocyte Lacunae, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct

    • Bo Wang, Lakiesha N. Williams, Amy L. de Jongh Curry, Jun Liao
    Pages 189-202

  19. Patch-Clamp Technique in ESC-Derived Cardiomyocytes

    • Jie Liu, Peter H. Backx
    Pages 203-214

  20. Optogenetic Control of Cardiomyocytes via Viral Delivery

    • Christina M. Ambrosi, Emilia Entcheva
    Pages 215-228
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Keywords

About this book

Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

Editors and Affiliations

  • Department Chemical Engineering&Applied Chemistry, University of Toronto, Toronto, Canada

    Milica Radisic

  • Department of Biomedical Engineering, Tufts University, Medford, USA

    Lauren D. Black III

Bibliographic Information

  • Book Title: Cardiac Tissue Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Milica Radisic, Lauren D. Black III

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1047-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1046-5Published: 29 July 2014

  • Softcover ISBN: 978-1-4939-4613-6Published: 24 September 2016

  • eBook ISBN: 978-1-4939-1047-2Published: 28 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 266

  • Number of Illustrations: 17 b/w illustrations, 60 illustrations in colour

  • Topics: Biotechnology, Regenerative Medicine/Tissue Engineering

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