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Expression of BMP and BMPRs in HepG2BRA and C2C12BRA cells. Total RNA from C2C12BRA and HepG2BRA cells was isolated and used as a template for the reverse transcriptase-polymerase chain reaction (RT-PCR) to examine the expression of BMP and BMPRs. G3PDH gene expression was used as an internal control. PCR products were separated in 1% agarose gel and visualized after ethidium bromide staining. (A) Representative RT-PCR analysis of total RNA using specific primers for BMP-2, BMP-4, BMP-6 and BMP-7. (B) Representative RT-PCR analysis of total RNA using specific primers for BMP type I receptors (ALK2, ALK3, ALK6) and BMP type II receptors (BMPR-II, ActR-IIA, ActR-IIB). Similar results were obtained in two separate experiments.
Zilberberg, Lior; ten Dijke, Peter; Sakai, Lynn Y; Rifkin, Daniel BJournal: BMC Cell Biology
Issue 1DOI: 10.1186/1471-2121-8-41Published: 2007-12-01Institution(s):
New York University School of Medicine, Leiden University Medical Center, Oregon Health & Science University and Shriners Hospital for Children
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