Dissection of Drosophila brain. (A) Sharpening of a Dumont no. 5 forceps. (B–D) Opening the head capsule, main steps (see
; steps 3 and 4 and Note 4). (E) Isolated brain with air sacs (arrows), posterior view. (F) Brain with partly removed air sacs, anterior view. (G) Dissected brain with air sacs completely removed; only some tracheae are still present (arrowheads).
They need to be sharpened with the help of sandpaper under a
dissecting microscope ( Fig.
Open up the head capsule from ventral to dorsal ( seeNote 4 ) by “cutting up” (i.e., carefully breaking the
cuticle with the two forceps; Fig.
Alternatively, make two further cuts through the cuticle of the
optic lobes ( Fig.
Once the brain is isolated, remove the esophagus, air sacs (
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