SpringerImages - Dependence of the number of 4D APSY-HNCOCA peaks of TM1290 calculated by GAPRO, P, on the value chosen for the GAPRO parameter Smin. Blue bars represent the number of identified correlation peaks, red bars the number of artifacts maintained in the final peak list and the dotted line is a reference indicating the expected 110 peaks

Dependence of the number of 4D APSY-HNCOCA peaks of TM1290 calculated by GAPRO, P, on the value chosen for the GAPRO parameter Smin. Blue bars represent the number of identified correlation peaks, red bars the number of artifacts maintained in the final peak list and the dotted line is a reference indicating the expected 110 peaks

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Fig 9

Dependence of the number of 4D APSY-HNCOCA peaks of TM1290 calculated by GAPRO, P, on the value chosen for the GAPRO parameter S_min. Blue bars represent the number of identified correlation peaks, red bars the number of artifacts maintained in the final peak list and the dotted line is a reference indicating the expected 110 peaks

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Figure 9 shows the variation of the peak list resulting from the 4D APSY-HNCOCA experiment of Figs. 3 and 4 when different values of S_min are used.

Application of the newly introduced GAPRO secondary peak filter had no impact on the final result obtained with S_min values in the range 5–8, which yielded a complete, artifact-free peak list from the 4D APSY-HNCOCA experiment of Figs. 3 and 4 (Fig. 9 ).

The potential impact is illustrated by its use, with n = 0 and R_single = 0 (Table 1 ), to validate the results obtained with S_min values of 3 and 4 (Fig. 9 ).

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