SpringerImages - Combinations of intraresidual and sequential correlations to be recorded with HN-detected APSY-NMR experiments used to collect the data needed for polypeptide backbone assignment of 13C,15N-labeled proteins. Each colored shape contains the nuclei correlated by a 4D or 5D experiment. The yellow areas contain the nuclei for which the individual correlations overlap. Chemical shift matching for these nuclei results in the generation of larger molecular fragments from the correlations obtained by the individual experiments. The notations used for the different groups of experiments reflect these matching chemical shifts, and indicate in parentheses additional important information for obtaining sequence-specific assignments (see text)

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Fig a

Combinations of intraresidual and sequential correlations to be recorded with H^N-detected APSY-NMR experiments used to collect the data needed for polypeptide backbone assignment of ¹³C,¹⁵N-labeled proteins. Each colored shape contains the nuclei correlated by a 4D or 5D experiment. The yellow areas contain the nuclei for which the individual correlations overlap. Chemical shift matching for these nuclei results in the generation of larger molecular fragments from the correlations obtained by the individual experiments. The notations used for the different groups of experiments reflect these matching chemical shifts, and indicate in parentheses additional important information for obtaining sequence-specific assignments (see text)

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