SpringerImages - Impact of the number of 2D projections recorded when using the three experiments 4D APSY-HACANH (magenta), 5D APSY-HACACONH (black) and 5D APSY-CBCACONH (red) for collecting the data needed to obtain sequence-specific polypeptide backbone resonance assignments for the protein TM1290 (see text). For each of the three experiments, the plot shows the percentage of the expected number of correlations, D, observed with a given number of 2D projection spectra, which were recorded in the order of the listing in Table

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Fig 11

Impact of the number of 2D projections recorded when using the three experiments 4D APSY-HACANH (magenta), 5D APSY-HACACONH (black) and 5D APSY-CBCACONH (red) for collecting the data needed to obtain sequence-specific polypeptide backbone resonance assignments for the protein TM1290 (see text). For each of the three experiments, the plot shows the percentage of the expected number of correlations, D, observed with a given number of 2D projection spectra, which were recorded in the order of the listing in Table

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In generating the Fig. 11 we made use of the fact that when using a combination of multiple experiments, such as the HA–CA(CB) group, the individual measurements can be recorded in an “interleaved fashion”, so that the number of 2D projections is augmented by one at a time across the group of experiments.

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