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Posterior aspects of fresh, coronal slices of two distinct hemispheres passing through the amygdala, as they appear on the cold plate (Fig. ), ready for the harvest of either the standardized (SBB) or additional blocks (ABB), and of the aliquots (vials). The Brodmann areas (BA) are shown color-coded (a) and are used as one of the identifiers of the blocks that include the cortex. The framed areas represent the standardized brain blocks SBB8 (amygdala), SBB7 (GP, globus pallidus), and SBB16 (cingulate gyrus) (b)
The banked fresh frozen samples consist of blocks or aliquots of
parenchyma obtained from precise anatomical areas, as defined by
Brodmann for the cerebral cortex (Figs. 9 , 10 ) [ 11 ].
Nonetheless, topographic accuracy of the standard blocks is secured
because of the distinctive condition of the slices obtained
(Fig. 10 )..
However, to ideally obtain the amygdaloid nucleus (SBB8), a cut
should pass through the posterior third of the anterior white
commissure (Fig. 10 b).
The standardization of the blocks is an integrative component of
the brain bank operation (Figs. 2 , 4 , 5 , 10 , 12 , 14 ).
Vonsattel, Jean Paul G.; Amaya, Maria Pilar; Keller, Christian E.Journal: Acta Neuropathologica
Issue 5DOI: 10.1007/s00401-007-0311-9Published: 2008-04-07Institution(s):
Columbia University, Columbia University Children’s Hospital
Carefully categorized postmortem human brains are crucial for research. The lack of generally accepted methods for processing human postmortem brains for research persists. Thus, brain banking is essential; however, it cannot be achieved at the cost of the teaching mission of the academic institution by routing brains away from residency programs, particularly when the autopsy rate is steadily decreasing. A consensus must be reached whereby a brain can be utilizable for diagnosis, research, and teaching. The best diagnostic categorization possible must be secured and the yield of samples for basic investigation maximized. This report focuses on integrated, novel methods currently applied at the New York Brain Bank, Columbia University, New York, which are designed to reach accurate neuropathological diagnosis, optimize the yield of samples, and process fresh-frozen samples suitable for a wide range of modern investigations. The brains donated for research are processed as soon as possible after death. The prosector must have a good command of the neuroanatomy, neuropathology, and the protocol. One half of each brain is immersed in formalin for performing the thorough neuropathologic evaluation, which is combined with the teaching task. The contralateral half is extensively dissected at the fresh state. The anatomical origin of each sample is recorded using the map of Brodmann for the cortical samples. The samples are frozen at −160°C, barcode labeled, and ready for immediate disbursement once categorized diagnostically. A rigorous organization of freezer space, coupled to an electronic tracking system with its attached software, fosters efficient access for retrieval within minutes of any specific frozen samples in storage. This report describes how this achievement is feasible with emphasis on the actual processing of brains donated for research.
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