SpringerImages - Double labeling of hippocampal and cerebellar cells with anti-GFAP antibody and anti-OPA1 antibody. A Hippocampal GFAP-positive protoplasmic astrocyte stained with fast red and DAB indicates that this GFAP-positive astrocyte expresses OPA1. B Cerebellar fibrillary astrocyte stained with DAB and fast red shows expression of OPA1 protein. The brown DAB staining indicates OPA1 labelling, the red staining indicates GFAP staining. The blue nuclei staining is due to a hemalaun counterstaining. Bars 50 µm

Caption

Double labeling of hippocampal and cerebellar cells with anti-GFAP antibody and anti-OPA1 antibody. A Hippocampal GFAP-positive protoplasmic astrocyte stained with fast red and DAB indicates that this GFAP-positive astrocyte expresses OPA1. B Cerebellar fibrillary astrocyte stained with DAB and fast red shows expression of OPA1 protein. The brown DAB staining indicates OPA1 labelling, the red staining indicates GFAP staining. The blue nuclei staining is due to a hemalaun counterstaining. Bars 50 µm

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Double-labeling with our OPA1 antiserum and a GFAP antibody showed double staining, indicating that there is also OPA1 expression in GFAP-positive astrocytes (Fig. 4 ).

Analyzing the cerebellum for glia cell labeling, we detected OPA1 expression in both protoplasmic astrocytes (Fig. 4 A) of the gray matter and in fibrous astrocytes of the white matter (Fig. 4 B)..

Using double-labeling techniques, we detected OPA1 protein expression in GFAP-positive astrocytes (Fig. 4 ).

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