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Confirmed isolates of Cronobacter spp. by biochemical testing (API 20E), chromogenic (α-MUG, DFI and EsPM), eight sets of Cronobacter spp. specific primers (α-GluA, α-GluB, SG, SI, Saka, OmpA, zpx and BAM) and 16S rRNA sequence analysis.
$On EsPM, colonies were blue black (BB) in chromogenic reaction color within 24 h at 37°C. €The PCR conditions for BAM primers as described in Table were used for amplification of both regions of the zpx gene as described by Kothary et al. . Analysis of the Cronobacter and non-Cronobacter strains was performed in a similar fashion. ¥ Vacuum dust. ND§: not determined. * Multiple bands. *#, PCR product was approximately (400 bp) and sequence was found not to be zpx. ##Colonies were blue black (BB) after three days at 37°C. £ Crono; Cronobacter spp.
sakazakii) (Tables 5 and 6) and thus were considered presumptive
were used to ascertain the identity of the isolates in this study,
only 13 isolates in addition to the ATCC (51329) strain were
positive with all the primers (Table 5).
As a result of final confirmation method only 29 isolates (Table 5)
were confirmed as Cronobacter spp.
Jaradat, Ziad W; Ababneh, Qotaiba O; Saadoun, Ismail M; Samara, Nawal A; Rashdan, Abrar MJournal: BMC Microbiology
Issue 1DOI: 10.1186/1471-2180-9-225Published: 2009-12-01Institution(s):
Jordan University of Science and Technology
This table is from the article titled "Isolation of Cronobacter spp. (formerly Enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, PCR and 16S rRNA sequencing"
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