Fig 2
Preparation of embryoid bodies by hanging drop method. (A) Generation of drops. (B) Plate with drops on inside of the lid.
Make 10-μL drops (10 3 cells per drop) on the inside of the lid using serial pipet ( Fig.
Carefully turn the lid around and fit it on the dish ( Fig.
Approximately 100 drops can be made in each lid ( Fig.
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