Sequence alignment of SoNCED and NCED proteins from several crop plants SoNCED is aligned with NCED proteins from Zea mays (Zm), Oryza sativa (Os), Citrus clementina (Cc) and Solanum lycopersicum (Sl). Dark backgrounds represent identical sequences, the underlined residues reflect the predicted chloroplast transit peptide of SoNCED, the number 1 under C410 and C430 indicates a di-sulfide bond, the triangles below the residues show identity for the His residues coordinated with the catalytic iron, and the alignment was performed using ClustalW2 and ESPript software
The SoNCED has been predicted to be located at chloroplast with a
chloroplast-targeting peptide at the N-terminus (Fig. 2).
Based on the deduced amino acid sequence alignment with the
sequences of other crop plants, the SoNCED showed high similarity
to the NCEDs of Zea mays, Oryza sativa, Citrus clementina and
Solanum lycopersicum (Fig. 2).
His residues in SoNCED (His-298, 347, 412, 590) were predicted to
be coordinated with catalytic iron for dioxygenase activity located
inside the β-propeller as in other NCEDs secondary structures
(Fig. 2; Messing et al.
Surface characteristic analysis of α1-helice and α3-helice revealed
a hydrophobic patch composed of 19 hydrophobic residues (Ala-95,
Ala-96, Ala-97, Ala-98, Ala-99, Leu-100, Ala-102, Val-108, Ala-109,
Val-111, Leu-112, Ile-229, Ala-230, Leu-233, Ala-234, Leu-235,
Ala-237, Ala- 239 and Ala-240) (Fig. 2) which were predicted
to penetrate the plastid membrane beyond the head groups of the
membrane phospholipids and interact with fatty acid residues (Tan
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