Human high-density chondrocyte pellets seeded with human respiratory epithelial cells and cultured in AECGM. The composite cultures were stained after 7 (a) and 14 (b) d with the antibody 34betaE12 (red), a marker for basal and goblet cells. Arrows devote epithelial cells attached to the surface of the pellet. No increase in hREC number could be detected from day 7 until day 14. The ECM of the pellets (x) was stained with alcian blue (asterisk).
In Fig. 3 , representative hREC-settled areas are
The number of epithelial cells, indicated through the diameter of
the epithelial layer, did not increase during the culture period
from days 7 (Fig. 3 a ) to 14 (Fig. 3
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