High levels of carbenicillin inhibited somatic embryo (SE) formation on embryogenic callus of ‘Doll’ horseradish. a ‘Doll’ horseradish embryogenic callus grown on MS with 2, 4-D (0.25 mg L−1) without carbenicillin formed a great number of globular SE-like structures (bar = 5 mm). b Embryogenic callus grown on induction medium with carbenicillin (2,000 mg L−1) formed fewer globular SE-like structures (bar = 5 mm)
At the highest dose of carbenicillin
(2,000 mg L−1) some leaf explants died early
in the experiment, survivors formed green nodular compact callus
with few globular globular SE-like structures (Fig. 1).
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