Maps of plant expression vectors. A pCAMBIA 1300, B pCAMBIA 1301, C pCAMBIA 1300-gus. LB Left border, RB right border, HPT hygromycin phosphotransferase gene, GUS β-glucuronidase gene, 35S pro 35S promoter of cauliflower mosaic virus, 35S poly A 3′ signal of 35S RNA, Nos terminator of nopaline synthase gene
The twin T-DNA vector pCAMBIA1300- gus was constructed
from the binary expression vector pCAMBIA 1301 and pCAMBIA 1300 (
www.cambia.org.au ); the pCAMBIA 1301 was excised with
Hind III and Xho I (blunted with T4 DNA polymerase) to delete the expression frame of
hpt gene and multiple cloning site and then circulated to
form plasmid pC1301-hyg − ; it was then digested with
Sac II and Sph I (blunted with Klenow) and
ligated into the Sac II (blunted) site of pCAMBIA 1300 to
generate the twin T-DNA plasmids vector pCAMBIA 1300- gus
(Fig. 1 ). .
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