SDS-PAGE and Western blotting analysis of rBLfA (a) and rBLfN (b) deglycosylation. M Protein Marker (kDa, TIANGEN, Beijing); M′ Prestained protein marker (kDa, TIANGEN, Beijing); (a) 1 and 1′ rBLfA treated with Endo H; 2 and 2′ untreated rBLfA; 3 and 3′ deglycosylation reaction buffer; (b) 1 and 1′ rBLfN treated with Endo H; 2 and 2′ untreated rBLfN; 3 and 3′ negative control, double distilled water treated with Endo H
After deglycosylation (Fig. 3), the apparent molecular
weight of rBLfA and rBLfN was decreased from 43.0 and 41.5 kDa
to 42.0 and 40.5 kDa which were close to the calculated
molecular weight of His-tagged-peptides rBLfA (41.972 kDa) and
rBLfN (40.666 kDa).
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