ImageJ analysis of the DGGE picture. Stations 1, 2 and 3 are panels A, B, and C respectively. The horizontal lines show the level of the background. The vertical lines show individual picks. A picture of the DGGE lanes is included next to the corresponding ImageJ analysis
ImageJ analysis (see Experimental procedures) of the DGGE banding
pattern shows less diversity in station 1 as compared to stations 2
and 3 (Fig. 1 ).
There were, however, two bands that migrated close to each other in
the three stations (2, 8, 5 and 3, 9, 6, respectively) (Fig.
This initial analysis suggested that there could be common
bacterial species among the three stations (Fig. 1 ). .
These results approximated the number of bands detected in DGGE
(Fig. 1 ).
Applying the ImageJ software analysis to our DGGE picture gave us
the number of bands per station (Fig. 1 ), where each band,
in theory, represents a single bacterial species.
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