Representative chromatograms of AA standard derivatives (a) and AA derivatives in healthy subject serum (b). Hypersil BDS C18 column: 4.6 mm × 200 mm × 5 m; column temperature 35°C; flow rate 1.0 ml/min; excitation and emission: λex/λem = 300/395 nm Peaks: 1 Arg, 2 Asp, 3 Ser, 4 Glu, 5 Thr, 6 Gly, 7 Ala, 8 GABA, 9 Pro, 10 Met, 11 Val, 12 Trp, 13 Phe, 14 Ile, 15 Leu, 16 (Cys)2, 17 His, 18 Orn, 19 Lys, 20 Tyr
We also found when the pH value of mobile phase A was 3.7, a
complete baseline resolution for 20 AA derivatives was obtained
within the shortest time and good peak shape (Fig. 3).
The representative chromatogram of AA in healthy subject is
presented in Fig. 3b.
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