Relative peak areas of a
LLYLR and b
YLR and photomodifications of these after UVA irradiation: 1 Y + O; 2 Y + 2O; 3 Y + O–2H; 4 Y + 2O–2H; 5 Y + N + 2O–H. Spectra peak areas are internally weighted, and the relative abundance of the peak area of the unmodified peptide at zero time is defined as 1.0. Error bars represent standard errors of the mean (±SEM)
Peptides containing tryptophan residues (Figs. 2, 3) were
observed to be modified considerably more rapidly than those
containing tyrosine residues (Figs. 4, 5), as evidenced
by greater and more definite decreases in parent peptides and
larger increases in modified products. .
This was demonstrated by the tyrosine degradation profiles;
sensitive MS/MS characterisation confirmed the presence of tyrosine
photoproducts (indicating the degradation of tyrosine residues),
whereas abundance profiling shows the majority of tyrosine
photoproducts to be present at very low abundance (evidenced by
maximum product levels only 10% as abundant as unmodified parent at
zero time, and by large measurement errors), to the point that most
are probably unsuitable for use as quantitative damage markers
using this technique (Figs. 4, 5).
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