RT-PCR assay of pPd9-1. a Ethidium bromide-staining gel showing amplified pPd9-1 bands (227 bp). Abbreviations are the same as Fig. . b Histogram of densitometry scan of pPd9-1 bands shown in a. The amount of each band was expressed as a relative amount; the intensity of the control of colony 1 was arbitrarily assigned a value of 1
An ethidium bromide-stained gel containing amplified RT-PCR product
is shown in Fig. 4 a.
pPd9-1 mRNA was detected in all treatments (the 227-bp bands in
Fig. 4 a), however, expression levels increased 2.3–5.9 fold
in the red soil treatment (Fig. 4 b).
In order to reject “false-positives”, we cloned the bands which
were present in both colonies, and one of the bands was confirmed
to be “true-positive” using RT-PCR (Fig. 4 ).
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