In situ localization of NO in the adventitious roots of mountain ginseng. NO was localized by staining with a specific dye DAF-2DA for NO (Kozima et al. 1998) in the adventitious roots subjected to the treatment of NO modulators viz. producer (SNP, e, h, f, i) or scavenger (CPTIO, b, f, i) in presence (g, h, i) or absence (d, e, f) of Cu. Adventitious roots were elicited for 24 h either with sterile water (control) or with 100 μM of SNP, 50 μM of CuSO4, 100 μM CPTIO, 100 μM sodium ferrocyanide alone or in combination as shown in figure. Scale bar = 200 μm
These results suggest that the presence of exogenous NO is
protective against oxidative damage induced by excess of Cu, while
higher NO concentration generated endogenously (Figs. 3 b, c,
4 g) due to Cu toxicity appears to be harmful.
1998 ) and observed under confocal laser scanning microscope as
green fluorescence (Fig. 3 c, Fig. 4 ).
Both SNP and excess of Cu were upregulated in situ NO content
(Fig. 3 c, Fig. 4 g).
Intense green fluorescence of NO was depleted on incorporation of
NO scavenger, the CPTIO (Figs. 3 c, 4 ) in growth medium.
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