SpringerImages - Conidiation defects of the ΔchsA ΔchsC double mutant are independent of genetic background. a Appearance of colonies of A237S/sC/T-1 (wild type, WT), A237SΔAC-1 (ΔchsA ΔchsC mutant, ΔAC), A237S/ΔA/T-1 (ΔchsA mutant, ΔA) and A237S/sC/ΔC-5 (ΔchsC mutant, ΔC). b mRNA levels of brlA and abaA. Total RNA was extracted from A237S/sC/T-1 (WT) and A237SΔAC-1 (ΔAC) cultured for 0 or 24 h on solid MMG after being transferred from liquid MMG. Probes specific to the genes indicated on the left were used. Ribosomal RNA (rRNA) stained with ethidium bromide is shown as loading control

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Fig 5

Conidiation defects of the ΔchsA ΔchsC double mutant are independent of genetic background. a Appearance of colonies of A237S/sC/T-1 (wild type, WT), A237SΔAC-1 (ΔchsA ΔchsC mutant, ΔAC), A237S/ΔA/T-1 (ΔchsA mutant, ΔA) and A237S/sC/ΔC-5 (ΔchsC mutant, ΔC). b mRNA levels of brlA and abaA. Total RNA was extracted from A237S/sC/T-1 (WT) and A237SΔAC-1 (ΔAC) cultured for 0 or 24 h on solid MMG after being transferred from liquid MMG. Probes specific to the genes indicated on the left were used. Ribosomal RNA (rRNA) stained with ethidium bromide is shown as loading control

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In contrast to the yellowish colonies of the wild-type, Δ chsA mutant, and Δ chsC mutant strains, the color of which resulted from the pigmented conidia, a newly derived Δ chsA Δ chsC double mutant (A237SΔAC mutant) formed whitish colonies, indicating that they produced few conidia (Fig. 5 a).

The mRNAs of brlA and abaA were not detected in A237SΔAC mycelia (Fig. 5 b).

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