SpringerImages - Expression of chsA::lacZ and chsC::lacZ in the brlA,abaA and medA mutants. a The wild type, brlA and abaA mutant strains were cultured in liquid MMG for 18 h at 37°C, and then placed onto solid MMG and cultured at 43.5°C. Mycelia were harvested at the indicated times and assayed for β-galactosidase activity as described in the text. b The wild-type and medA mutant strains were cultured in liquid MMG for 18 h at 37°C, and then placed onto solid MMG and cultured at 25°C

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Fig 1

Expression of chsA::lacZ and chsC::lacZ in the brlA,abaA and medA mutants. a The wild type, brlA and abaA mutant strains were cultured in liquid MMG for 18 h at 37°C, and then placed onto solid MMG and cultured at 43.5°C. Mycelia were harvested at the indicated times and assayed for β-galactosidase activity as described in the text. b The wild-type and medA mutant strains were cultured in liquid MMG for 18 h at 37°C, and then placed onto solid MMG and cultured at 25°C

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To examine this possibility, we constructed brlA , abaA , and medA mutants, in which one of two fusion constructs was expressed [ chsA (p/l):: lacZ or chsC (p/l):: lacZ ](see Materials and methods), and determined expression levels of chsA and chsC by measuring β-galactosidase activities (Fig. 1 ).

The chsC :: lacZ expression in the wild-type strain started to increase during 10–20 h after developmental induction, and reached a maximum level at 30-h postinduction (Fig. 1 a).

The chsC :: lacZ expression in the wild-type strain peaked at 50-h postinduction, then declined until 100 h (Fig. 1 b).

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