Electropherograms of human plasma spiked with 1 μg mL−1 INH. a Sweeping MEKC: sample matrix, 75 mM phosphoric acid; sample injection scheme, 200 mbar for 1.5 min; BGE, 50 mM phosphoric acid, 100 mM SDS, and 15% ACN; separation voltage, −20 kV. b CSEI-Sweep-MEKC: sample matrix, 30 mM phosphoric acid and 50% MeOH; sample injection scheme, 8 kV for 360 s; NMBGE, 75 mM phosphoric acid and 15% ACN; HCB, 200 mM phosphoric acid, 30 mbar for 2 min; water plug, 20 mbar for 0.1 min; MBGE, 50 mM phosphoric acid, 100 mM SDS, and 15% ACN; separation voltage, −20 kV
The samples were pretreated with SPE as described in “Sample
cleanup procedure.” As shown in Fig. 2a, endogenous
interferences gave a complicated electropherogram in sweeping MEKC
analysis, which resulted in low quantification accuracy when INH is
in low concentration.
In contrast, the electropherogram obtained from CSEI-Sweep-MEKC was
relatively clean (Fig. 2b).
Other conditions are the same as those described in Fig. 2 .
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