Electropherograms of human plasma spiked with 1 μg mL−1 INH. a Sweeping MEKC: sample matrix, 75 mM phosphoric acid; sample injection scheme, 200 mbar for 1.5 min; BGE, 50 mM phosphoric acid, 100 mM SDS, and 15% ACN; separation voltage, −20 kV. b CSEI-Sweep-MEKC: sample matrix, 30 mM phosphoric acid and 50% MeOH; sample injection scheme, 8 kV for 360 s; NMBGE, 75 mM phosphoric acid and 15% ACN; HCB, 200 mM phosphoric acid, 30 mbar for 2 min; water plug, 20 mbar for 0.1 min; MBGE, 50 mM phosphoric acid, 100 mM SDS, and 15% ACN; separation voltage, −20 kV
The samples were pretreated with SPE as described in “Sample
cleanup procedure.” As shown in Fig. 2a, endogenous
interferences gave a complicated electropherogram in sweeping MEKC
analysis, which resulted in low quantification accuracy when INH is
in low concentration.
In contrast, the electropherogram obtained from CSEI-Sweep-MEKC was
relatively clean (Fig. 2b).
Other conditions are the same as those described in Fig. 2 .
Viewing this image requires a subscription. If you are a subscriber, please log in.
This image is from the article titled "Quantitative determination of isoniazid in biological samples by cation-selective exhaustive injection–sweeping–micellar electrokinetic chromatography"
(from Analytical and Bioanalytical Chemistry), which is copyrighted by Springer-Verlag. For more information on the
copyright for this image, please refer to the full image caption and to the
The image is being made available for non-commercial purposes for subscribers to SpringerImages. For more information on what you are allowed to do with this image, please see our copyright policy.
To request permissions to use any copyrighted material, please visit the source document.
Report a copyright concern regarding this image.
Log in or register to save your favorite images and download them as high-quality PowerPoint or PDF files.
Log in or register to save your search criteria.
© Springer, part of Springer Science+Business Media.
Remote Address: 18.104.22.168 Server: 19