SpringerImages - Scanner image and signal computation, showing the intra-spot signal distribution from a deposited droplet containing 10 mg mL–1 BSA-Cy3, which was immobilized on an aldehyde-functionalized surface and analyzed as “dried” after blocking and washing. The 3D surface contour plot represents the morphology of the spot signal intensity (Software: Image J, Interactive 3D surface plot)

Scanner image and signal computation, showing the intra-spot signal distribution from a deposited droplet containing 10 mg mL–1 BSA-Cy3, which was immobilized on an aldehyde-functionalized surface and analyzed as “dried” after blocking and washing. The 3D surface contour plot represents the morphology of the spot signal intensity (Software: Image J, Interactive 3D surface plot)

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Fig 2

Scanner image and signal computation, showing the intra-spot signal distribution from a deposited droplet containing 10 mg mL^–1 BSA-Cy3, which was immobilized on an aldehyde-functionalized surface and analyzed as “dried” after blocking and washing. The 3D surface contour plot represents the morphology of the spot signal intensity (Software: Image J, Interactive 3D surface plot)

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Figure 2 shows the homogeneous morphology of a single spot of Cy3-BSA printed under fluorocarbon as a 3D contour surface plot representing the distribution of fluorescence intensity.

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