Immunohistochemical staining of retinal cryo-sections with antibodies against neuronal (a), endothelial (b), and inducible (c) nitric oxide synthases. Layers: g.c.l. ganglion cell layer, i.p.l. inner plexiform layer, i.n.l. inner nuclear layer, o.p.l outer plexiform layer, o.n.l. outer nuclear layer, o.p.s. outer photoreceptor segments, r.p.e. retinal pigment epithelium. Scale bar 50 µm
Figures. 4 a–c show immunoreactivity of neuronal, endothelial
and inducible NOS, respectively, in control and axotomised retinas
at different time points.
Neuronal NOS immunoreactivity is visible, in particular, in the
inner nuclear layer, where amacrine and bipolar cells are located
(Fig. 4 a).
in the outer part of the inner plexiform layer and in the outer
plexiform layer (Fig. 4 b).
Inducible NOS immunoreactivity is scattered densely across the
inner and outer nuclear layers (Fig. 4 c).
In particular, no axotomy-induced increase in neuronal NOS as
reported by Koeberle and Ball [ 41 ] could be found in our study
(Fig. 4 a).
Moreover, inducible NOS immunoreactivity in microglial or Müller
cells after an axotomy, as described by these authors, could not be
detected unambigously here (Fig. 4 c).
Viewing this image requires a subscription. If you are a subscriber, please log in.
This image is from the article titled "Measurement of nitric oxide production by the lesioned rat retina with a sensitive nitric oxide electrode"
(from Analytical and Bioanalytical Chemistry), which is copyrighted by Springer-Verlag. For more information on the
copyright for this image, please refer to the full image caption and to the
The image is being made available for non-commercial purposes for subscribers to SpringerImages. For more information on what you are allowed to do with this image, please see our copyright policy.
To request permissions to use any copyrighted material, please visit the source document.
Report a copyright concern regarding this image.
Log in or register to save your favorite images and download them as high-quality PowerPoint or PDF files.
Log in or register to save your search criteria.
© Springer, part of Springer Science+Business Media.
Remote Address: 126.96.36.199 Server: 20