Immunohistochemical staining of retinal cryo-sections with antibodies against neuronal (a), endothelial (b), and inducible (c) nitric oxide synthases. Layers: g.c.l. ganglion cell layer, i.p.l. inner plexiform layer, i.n.l. inner nuclear layer, o.p.l outer plexiform layer, o.n.l. outer nuclear layer, o.p.s. outer photoreceptor segments, r.p.e. retinal pigment epithelium. Scale bar 50 µm
Figures. 4 a–c show immunoreactivity of neuronal, endothelial
and inducible NOS, respectively, in control and axotomised retinas
at different time points.
Neuronal NOS immunoreactivity is visible, in particular, in the
inner nuclear layer, where amacrine and bipolar cells are located
(Fig. 4 a).
in the outer part of the inner plexiform layer and in the outer
plexiform layer (Fig. 4 b).
Inducible NOS immunoreactivity is scattered densely across the
inner and outer nuclear layers (Fig. 4 c).
In particular, no axotomy-induced increase in neuronal NOS as
reported by Koeberle and Ball [ 41 ] could be found in our study
(Fig. 4 a).
Moreover, inducible NOS immunoreactivity in microglial or Müller
cells after an axotomy, as described by these authors, could not be
detected unambigously here (Fig. 4 c).
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