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Procedures of clone screening after RHKD. RHKD tet-inducible lentiviral vector design (top) and screening procedure of 3T3-L1 cells for adipogenesis with RHKD. CMV/TRE, cytomegalovirus/tetracycline response element; GFP, green fluorescent protein; PGK, phosphoglycerate kinase promoter; Blast, blasticidin-S deaminase for selection against blasticidin after lentiviral infection; LTR, long terminal repeat. Left microphotograph shows a clone of 3T3-L1 cells (GFP-positive) after RHKD, which have little lipid accumulation inside the cells, surrounded by non-infected (GFP-negative) and properly differentiated 3T3-L1 adipocytes. Right microphotograph shows a clone of 3T3-L1 cells (GFP-positive) after RHKD, which have over accumulated lipid inside the cells, compared to surrounding non-infected (GFP-negative) and properly differentiated 3T3-L1 adipocytes.
1, Tet-on 3T3-L1 cells were infected with RHKD lentivirus at an
infection rate of <10% in order to obtain single copy
Niimi, Masashi; Tao, Lian; Lin, Shi-Hua; Yin, Jun; Wu, Xiaoyun; Fukui, Hiroyuki; Kambayashi, Junichi; Ye, Jianping; Sun, BingJournal: Journal of Biomedical Science
Issue 1DOI: 10.1186/1423-0127-16-92Published: 2009-12-01Institution(s):
Otsuka Maryland Medicinal Laboratories Inc, University of Tokushima, Louisiana State University, University of Alabama at Birmingham School of Medicine
This image is from the article titled "Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells"
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