Human dendritic cells grown in plastic cell culture flasks were incubated with human T-cells. At different time points samples were fixed and prepared for field emission scanning electron microscope (FESEM) image analysis. The T-cell (red) induced membrane-ruffle on the DC-cell (yellow) engulfs the entire T-cell. By live-video microscopy it was shown that this interaction lasts for about 3-4 minutes and then the T-cell is released from the engulfing membrane. DC's were derived from human samples, were allogenic and expression profiles of DC markers were tested by fax scan to make sure that they were truly DC's. The T-cell population was also tested before introduction to the DC's. Evidence for the cross-talk was brought by live imaging movies of the DC/T-cell interactions which were acquired before the FESEM studies were set up. After fixation with formaldehyde and glutaraldehyde, samples were dehydrated with acetone, critical-point dried and sputter coated with gold before examination in the FESEM. Colorized in Adobe Photoshop CS. Bar represents 2 µm.

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