Escherichia coli colony pattern
The image shows the colony pattern that emerges during the E.
coli bacteria development on a semisolid surface after 1 week
MG1655 E. coli K-12 strain was cultivated overnight in an
Erlenmeyer flask containing 10 ml of Luria Bertani (LB, 1% Difco
bacto-tryptone, 0.5 % Difco yeast extract, 0.5 % NaCl) broth at
37ºC until the stationary-phase of growth was reached. A 2.5 µl
aliquot of this culture was inoculated on the semisolid surface of
a Petri swarming plate: 1% Difco Bacto-tryptone, 0.5 % Difco Yeast
extract, 0.5 % NaCl. The liquid medium was harnessed with 1% Difco
agar to create a semisolid gel. The plate was incubated in an oven
for 72 hours at 30ºC. After this time the plate was mantained a
week at room temperature and then was photographed with
epilumination using an Olympus CCD device DP70 camera fitted with
an optical zoom. The total size of the colony image is 3.5
For more information see Zorzano et al. (Phys. Rev. E 2005) and
et al. (BMC Biol. 2007).
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Copyright: José María Gómez-GómezCredit: José María Gómez-GómezContributed by: José María Gómez Gómez
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