Height and phase AFM images of the isolated plasma membrane of the prestin-transfected CHO cell incubated with antiprestin antibody and Qdot-conjugated secondary antibody. a High-magnification height image. Different sizes of particles are observed (A and B, arrows). b Phase image of a. c Cross sections of phase images of the particles A and B (between α and α′ and β and β′, arrowheads). Regarding particle B, a bright phase contrast (positive phase shift) was observed at the center of the particle while a dark phase contrast (negative phase shift) was observed at its periphery. By contrast, such phase shifts were not observed for particle A. Scale bars are 300 nm
Figure 7 a shows a high-magnification AFM height image of the
isolated plasma membrane of the prestin-transfected CHO cell, the
prestin molecules of which were labeled with Qdots.
Figure 7 b shows the phase image of a.
Figure 7 c indicates the cross sections of the phase images of
particles A and B obtained between α and α′, and β and β′
Although several sizes of particles on the plasma membrane were
observed in the height mode (A and B, Fig. 7 a), only
particle B, approximately 8 nm in height, showed a phase
shift, that is, the bright phase contrast (positive phase shift)
was observed at the center of the particle, whereas the dark phase
contrast (negative phase shift) was observed at its periphery,
suggesting that this particle was a Qdot..
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